Proton turnover rates of cytochrome bo3 ubiquinol oxidases measured at the single-enzyme level
Principal Investigators: Dr. Stephan Block (FU)
A microscopy-based single-liposome assay is used to quantify proton turnover rates of cytochrome bo3 ubiquinol oxidases on the single-enzyme level. Proton turnover will be studied in dependence of various parameters, such as the curvature and lipid composition of the environment used for enzyme reconstitution. Microfluidics will be used to control the rate of electron delivery towards the enzyme, aiming to operate the enzyme at low turnover rates and therefore probing the tightness of the coupling between electron transfer and proton translocation at in vivo-relevant low electron delivery rates.